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To determine reference ranges, labs test the blood of a large representative population sample (chosen based on characteristics like age, sex, and general health status) and use statistical analysis to identify the middle 95 percent of values for the nutrient in question

Hold for 30 seconds and repeat three times

Compare with a control solution prepared using 5.0mL of standard sodium chloride solution

doi: 10.1097/00001813-200211002-00002 115 BorossPLeusenJH