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After five washing steps with PBS-Tween, the secondary antibody (Alexa Fluor 555 anti-mouse, Life Technologies, 1:200) was added in 2% BSA/PBS-Tween together with Hoechst 33342 (1 g/ml) for 2 h
Topical antibiotics or antifungal eye drops are the usual treatment for corneal abrasions in order to avoid subsequent infection
Nrf2, a pivotal transcription factor responsible for maintaining cellular metabolism and redox balance, governs various molecules associated with ferroptosis, including FT light and heavy chains (FTL/FTH1), the system Xc subunit, the catalytic and regulatory subunits of glutamic acid cysteine ligase (GCLC/GCLM), as well as GPX4 [64, 65]
When Progress Stalls A short troubleshooting ladder for the research setting identify the bottleneck, its likely cause, and the studied adjustment
Allergic reactions are rare but documented
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