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Nat Rev Immunol 6 , 358370 (2006)

Results 3D gels selectively support the survival of a subset of lymphoid tumor cells While growth rates of LCLs (X50-7 and JY) and lymphoma B cells (DG-75 and BL-2) in 2D culture conditions are nearly identical [28], lymphoma B cells form colonies within 3D gels, unlike LCLs (Fig

However, significant loss of potency can occur with no visible changes, so you must rely on proper storage protocol, not just sight