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The enzymatic reaction was initiated by adding 1 mL of GPx enzyme solution (3000 units/liter) to 9 mL of a mixture containing potential interfering substances
151 LiCHShyuMKJhanCChengYWTsaiCHLiuCWet al
With their own value of 240 mV, which was based on the spontaneous equilibration with the NADH/NAD redox couple, they nicely comply with the E m7 which is at present dogmatically accepted, although method sensitivity remains a problem (Van Laer et al., 2013)
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