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After 24 h cells were collected by trypsination, counted, and washed with cold PBS

Human trial data is limited to a handful of small pilot studies, and a 2025 University of Utah review concludes BPC-157 should be considered investigational

Then add escalation, missed-dose, measurement, monitoring, and red-flag context

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For instance, it can be hypothesized that in a PIK3CA-mutant EAOC cell, a concurrent ER-mediated repression of the remaining wild-type PTEN allele would ensure maximal pathway activation