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Heliyon 10(3):e24943

For transient expression, cells in 12-well plates were typically transfected at roughly 70% confluency using 2.5 l of polyethyleneimine (PEI

Glutathione helps recycle these molecules, bringing them back into function and extending their ability to attack free radicals: for this reason Glutathione is considered the "master" or "King" molecule of all the antioxidants in the body

They had identified 5-hydroxytryptophan and kynurenine as the reaction products by matching the retention times with their authentic samples by performing high-performance liquid chromatography (HPLC) analysis 24

Liu L, Miao Y, editors