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In vitro cellular senescence models (such as senescence models of human fibroblasts, hepatocytes, and nerve cells) are common tools for studying aging mechanisms and interventional drugs

Mutation Research/Genetic Toxicology and Environmental Mutagenesis 774:816

If you're noticing significant immediate effects, placebo response may be contributing, which isn't necessarily bad but shouldn't set unrealistic expectations for continued dramatic improvements

Depletion of MSCs and inefficient cell signaling can be detrimental to aging changes

Other contributing factors include prolonged sun exposure and genetic predisposition