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The remaining rudimentary sclerotin of the mandible was thin and so a titanium plate was used for structural reinforcement (Fig
Protein purification under denaturing conditions: Proteins that are expressed as an inclusion body and have been solubilized with chaotrophic agents such as guanidine-HCl or urea can be purified by modifying the protocol to include the appropriate amount of denaturant (up to 6M guanidine-HCl or up to 8M urea) in the binding, wash and elution buffers
doi: 10.3892/mmr.2019.10499 213 YangQYYangKPLiZZ
