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Biochem Biophys Res Commun 242: 19
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One hour before the addition of calcium/phosphate/DNA mixture, conditioned medium was removed and kept in a 10% CO 2 incubator at 37 C, and serum-free Dulbeccos modified Eagle medium (DMEM) with high glucose (Invitrogen, Catalog No.12100046) without antibiotics that had been pre-warmed in a 10% CO 2 incubator at 37 C was added to the cultured cells
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