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Following block in 5% milk or BSA, membranes were incubated with an appropriate primary antibody from Cell Signaling (Danvers, MA) against pAkt Ser473 (1:1000

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The specific day is flexible, but be sure to rotate injection sites between the abdomen, thigh, or upper arm to avoid irritation

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This can largely be attributed to a loss of exclusivity for their multiple myeloma drug Revlimid (lenalidomide)