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Eur J Endocrinol 143 , 585592
Destined exclusively for in vitro research and preclinical studies on non-human models
Interestingly, MDA concentration increases significantly in acute asthma, which suggests that peroxidation processes are intensified during disease progression (89)
cAMP accumulation assay Wild-type GLP-1R and GLP-1R mutants were cloned into the expression receptor pcDNA3.1/V5-His-TOPO vector (Invitrogen) at the HindIII and EcoRI sites by using the QuickChange site-directed mutagenesis and a flag-tag was inserted after native signal sequence (see primers in Supplementary Table 2)
Detailed analyses of kinetic parameters showed that PFK depletion and, thus, FBP reduction decreased the rate of FA assembly and reduced the generation of FAs (Fig
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