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Description

For real-time calcium imaging assay, plates were incubated at 4 C for 2 days in the dark and then transferred to 22 C with a SD photoperiod for 67 days

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GSH was measured by adding the standard or sample to 100 L of a 1:1 mixture of 3 units/mL glutathione reductase with 0.67 mg/mL 5,5'-Dithiobis(2-nitrobenzoic acid) (DTNB)