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First, to a 1.5-mL Eppendorf tube were added 25 L of GSH (12 mM), 45 L of CDNB (3.0 mM), 50 L of GST (appropriate concentration), and 200 L of triethanolamineHCl buffer (20 mM, pH 6.5), and then, the mixed solution was incubated for 60 min at 25 C
Immunofluorescence staining The paraffin-embedded sections were deparaffinized and subjected to antigen retrieval in a boiling sodium citrate solution (0.01 mol/L, pH 6.0)
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Compared with those using insulin, people using a GLP-1 receptor agonist had a lower risk for developing gallbladder cancer (HR = 0.35
A systematic review and meta-analysis demonstrated that pacing interventions lead to a significant reduction in fatigue (Hedges g = -0.52) and an improvement in physical function among ME/CFS patients compared to no treatment or routine care [175]
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