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Preparation of hypothalamic samples for LC-MS/MS analysis For the proteomic analysis by LC-MS/MS, hypothalamic samples were dissected, immediately frozen in liquid nitrogen, and stored at 80 C until used

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Am J Med 105:54S-58S Tomassini V, Pozzilli C, Onesti E, Pasqualetti P, Marinelli F, Pisani A, Fieschi C (2004) Comparison of the effects of acetyl l-carnitine and amantadine for the treatment of fatigue in multiple sclerosis: results of a pilot, randomised, double-blind, crossover trial