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Protective Effect of Glutathione against Oxidative Stress-induced Cytotoxicity in RAW 264.7 Macrophages through Activating the Nuclear Factor Erythroid 2-Related Factor-2/Heme Oxygenase-1 Pathway

Accutane is a medicine extracted from Vitamin A that operates by lowering the size and actions of sebaceous glands in the skin

Oil Red O staining 3T3-L1 cells were washed with PBS twice, fixed with 10% formalin at room temperature for 10 min, and stained with Oil Red O (Sigma) at 37 C for 10 min

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Finally, the cells were exchanged with pre-warmed FBS-free DMEM medium, and then the DNA-PEI mixture was added to the cells gently