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Further, as TP53 heterozygous deletions often co-occur with RPS15 mutation, we intercrossed Cd19 -Cre/ Rps15 MT mice with Trp53 heterozygously-deleted mice ( Trp53 del ) to generate Trp53 del / Rps15 MT co-mutant murine cohorts (Methods)

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The retentate was suspended in PBS and then re-centrifuged

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