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Blots were probed with the indicated antibodies, and protein bands were visualized using Chemi-Lumi One L (07880-70, Nacalai Tesque) or Immobilon Western Chemiluminescent HRP substrate (WBKLS0500, Millipore)

This cannot be established through clinical assessment alone and requires a scan

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Pregnant or breastfeeding women should talk to their doctor before getting B12 injections