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Sex was not considered as a biological variable in the primary study design because (i) cyclical hormonal variation in female mice is a known confounder of LPS-induced inflammatory cytokine responses, and (ii) the C57BL/6J LPS doseresponse and time course on which our paradigm was based has been characterized primarily in males in prior literature
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If you notice any cloudiness, discoloration, or visible floating particles, its a strong indicator of degradation or bacterial contamination, and the vial should be safely discarded
A lower-dose formulation (Alli 60 mg) is available over the counter for adults with a BMI of 28 kg/m or above
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