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Real-time PCR Total RNA was isolated from the cells using the Roche High Pure RNA isolation kit (Roche Life Science, 11828665001) and reverse transcription was performed using the iScript cDNA synthesis kit (Bio-Rad, 1708891) according to the manufacturers instructions

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Explore the brain scan differences DMN: The Rumination Network and Maladaptive Prospection How the default mode network drives maladaptive prospection, chronic rumination, and its metabolic cost