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Particle auto-picking was performed using a pretrained model in the crYOLO software package, followed by particle extraction in a box-size equivalent to 320 and Fourier down-sampled to 64 pixels

Amplification products were examined for nonspecific amplification by including an additional denaturation step in the real-time thermal cycler protocol

Avoid heavy, fatty, or spicy foods immediately before or after injection, as these can intensify gastrointestinal symptoms

Even modest improvement in fitness can yield outsized longevity resultsmaking this the single most important vital sign after blood pressure