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The original activity associated with GIP was the inhibition of gastric acid secretion and the protein was originally called gastric inhibitory peptide
the addition of sulphane sulphur) with the concomitant functional and/or structural consequences (reviewed e.g
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The protein was then incubated overnight with TEV protease on the column to remove the C-terminal 2MBP-tag in the buffer above at 4 C
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