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A study by Cummings et al (2000) on isolated mitochondria from cells lacking porins indicated that in this invitro setting porins do not contribute to glutathione transport to the mitochondrial matrix which might be due to a rate limiting transport of glutathione over the IMM (see Figure 4)
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Take 1 mL of the extract into a 15 mL test tube, add 9 mL of acetonitrile, and vortex mix for 1 min
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