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Time-to-onset analysis Time-to-onset was calculated as the duration between GLP-1 RA initiation and the occurrence of a NAE 21
At each time point, 1/10 volume of culture medium per well CCK-8 solution reagent was added, incubated at 37C for 3 h, and the absorbance was measured at 450 nm using a microplate reader
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Dry skin types can add a moisturizer on top for extra hydration
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