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Isolated mitochondria were washed twice with basic STE buffer (without 0.1% fatty acidfree BSA), resuspended in basic STE buffer and protein concentration of mitochondrial proteins was determined by the bicinchoninic acid (BCA) assay using BSA as a standard

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doi: 10.1097/FJC.0000000000001299 108 De BlockCBaileyCWyshamCHemmingwayAAllenSEPeleshokJ

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Transneuronal propagation of pathologic alpha-synuclein from the Gut to the brain models parkinsons disease