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The animals were randomly divided into six experimental groups (six rats/group): CTRL group (rats were injected intraperitoneally with saline on day 7 plus Kolliphor EL from day 1- day 10), MTX group (rats were injected MTX intraperitoneally on day 7 plus Kolliphor EL throughout the experiment), NTZ-100 and NTZ-200 groups (rats were injected intraperitoneally with MTX on day 7 plus NTZ (100 and 200 mg/kg, daily) from day 1- day 10 by oral gavage, respectively, plus Kolliphor EL from day 1- day 10) (Elaidy et al., 2018