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Assuming a consistent protocol and, crucially, some form of UV stimulation, this is the period where tangible, observable results begin to appear

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Often possible, but dehydration from GI side effects can worsen kidney function, so monitoring and hydration are important

Most proteins are not stable in acidic pH, so you will want to adjust your pH back to the desired value using a concentrated base such as sodium hydroxide before using your elution buffer

If you need long-term storage exceeding refrigerator timeframes, dedicated freezer storage at -20C is appropriate for lyophilized peptides