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Cells of 2 L culture were resuspended in buffer 1 (100 mM HEPES-NaOH pH 7.5, 10 mM MgCl 2 ,150 mM NaCl) or in buffer 1 containing 50 mM NaCl, supplemented with 2.5 units/mL Turbo Nuclease (Jena Bioscience) and disrupted by a single passage through a french press at 14,500 psi
Gopal GJ, Kumar A
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Chronic fatigue and fibromyalgia : Anecdotal reports suggest symptom improvement in these conditions, though there is no official link established through rigorous clinical trials
Because the peptide chain is relatively long compared with some signaling fragments, careful storage is essential
Riboli E, Norat T
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