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When cells reached 80% confluence, the monolayer was rinsed 3x with PBS and cultured in phenol red-free MEM without additives (no FBS, L-glutamine, ITS, or estradiol)
Sequential changes in trace metal, metallothionein and calmodulin concentrations in healing skin wounds
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Abdominal bloating decreases (partly from visceral fat reduction, partly from reduced inflammation in adipose tissue)
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