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The NHS advises that taking up to 2mg (2000 micrograms) daily of vitamin B12 supplements is unlikely to cause harm, with no established upper limit in the UK
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p53 TAD (13-61) was subcloned into the pET His 6 glutathione-S-transferase (GST) TEV LIC cloning vector (2G-T, obtained from Scotta Gradia, Addgene plasmid #29707) and transformed into BL21 (DE3) pLysS cells
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