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Community-derived planning most often references 4-6 week cycles, with extended 6-8 or 8-12 week cycles for slower-remodeling tissue research models

Under sub-optimal conditions warm ambient temperatures, frequent puncture without consistent aseptic technique, light exposure the effective preservation window may shorten to less than 28 days even though the calendar window has not elapsed

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Changes to the microbial phyla and genera observed in the 5A-1MQ-treated group switched from WD to LD could have been driven by additive, synergistic, or independent mechanisms relative to those induced by diet switch alone, which could not be determined with the current study design that lacked a group treated with 5A-1MQ and maintained on WD