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European Bioinformatics Institute

A semi-automated method for the measurement of ATP and 2,3DPG in the supernatant, obtained by the addition of an equal volume of trichloroacetic acid (80 g/L) to whole blood or washed erythrocytes in isotonic saline (to precipitate protein) and centrifugation of the mixture (Godwin et al ., 1983), has been used for measurement of these substances in a wide range of Australian mammals