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Briefly, neutral and polar fatty acids were analyzed following a three-phase liquid extraction 107

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The mobile phase consisted of 0.1% formic acid in water (A) and methanol (B), with a gradient elution: 2% B to 30% B over 30 min, then to 60% B in 8 min, to 100% B in 2 min (hold 5 min), and re-equilibration to initial conditions in 1 min

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10.1016/j.taap.2005.01.061 91