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While singly-housed animals may experience stress that impacts metabolism, body weight, and adiposity 65,66,67,68,69 , the singly-housed animals in this study were treated identically (to the best of the experimenters ability) to minimize and equalize stress across all animals and reduce confounding variables

Rest and relax as much as possible

Enzyme-linked immunosorbent assay kits were used to determine the levels of pro-inflammatory cytokines, specifically TNF- and IL-6, in cell culture supernatants

May Help Reduce Inflammation Inflammation can contribute to pain, delayed healing, and reduced mobility

Bone is constantly renewing itself