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To perform double immunofluorescence staining, brain sections were incubated using the primary antibody of vWF (1:100, FITC, Abcam, Cambridge, MA, United States) and anti-GLP-1R (1:50, sc-390774, Santa Cruz Biotechnology Inc.) overnight at 4C
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I had never tried GLP-1 treatment before, so before purchasing Lenreey , I did a lot of research and read through several case studies
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Berberine, conversely, works primarily through AMPK activation and multiple downstream pathways
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