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[31] [28] Peptides are distinguished from traditional small molecules by their unique combination of residue sequence, amide backbone flexibility, and susceptibility to chemical modifications, all of which dictate bioavailability and membrane permeability

This typically resolves within 24-48 hours and can be minimized by proper injection technique and site rotation

Lyophilized (freeze-dried) peptides may vary in appearance from fine loose powder to compact solid pucks

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Reversed-phase HPLC separates target peptide molecules from synthesis residues, optical isomers, or sequence fragments