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Use sterile handling, avoid vigorous shaking and repeated freeze-thaw cycles, and do not use the material if the seal is damaged or if persistent particles, unexpected cloudiness, or discoloration is present after dissolution
The modification level of m 6 A was determined as the percentage of m 6 A out of the total amount of A, to normalize the amount of mRNA injected from different samples
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F., Guo, C
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