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We identified six biophysical properties that can be predicted from a sequence using the ProtParam module ( from the biopython Python package 45 : (1) the isoelectric point in neutral pH, (2) GRAVY (grand average of hydropathy) 46 , (3) the instability index 47 , (4) aromaticity, (5) the molar extinction coefficient and (6) molecular weight
When performing an intention-to-treat analysis, the HR for MOF was found to be 0.89 [0.781.02] with GLP-1 RAs compared to DPP-4is, although this became significant in the fully adjusted model (HR 0.85 [0.74 0.98])
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