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Small interfering RNA (siRNA) transfection assay Primary hepatocytes were transfected with siRNA to knockdown p53, p21, and progerin, according to the manufacturer instructions

LC-MS measurement of HepG2 proteome Peptides were measured and online-separated using an UltiMate 3000 nano HPLC system ( Dionex ) coupled to a Bruker timsTOF Pro mass spectrometer via a CaptiveSpray nano-electrospray ion source and Sonation column oven

If the solution seems thicker when drawn into a syringe compared to when it was fresh, aggregation has likely progressed substantially

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i , Immunoblot of indicated proteins in HEK-293T cells expressing an SLC25A40 cDNA in the presence or absence of BSO