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Protein expression was induced with 0.3 mM isopropyl -D-1-thiogalactopyranoside (IPTG) when the culture reached an optical density (O.D.) of 0.6

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Safety concerns included uncharacterized immunogenicity risks, inadequate impurity and endotoxin testing in available raw material, the unresolved free-base versus acetate salt form distinction, and, for BPC-157 specifically, an angiogenesis mechanism that raises theoretical concerns for patients with undiagnosed tumors

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