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The effects of the mutation are reduced by treatment with NACA, resulting in increased survival of Schwann cells
2D two-dimensional hiPSC human induced pluripotent stem cell 3D three-dimensional hPSCs human pluripotent stem cells AAV adeno-associated virus HUVECs human umbilical vein endothelial cells ADPKD autosomal dominant polycystic kidney disease iETV2-hiPSCs inducible ETV2-expressing human induced pluripotent stem cells AI artificial intelligence iPSC induced pluripotent stem cell AKI acute kidney injury NF-B nuclear factor kappa-light-chain-enhancer of activated B cells ALI air-liquid interface NGAL neutrophil gelatinase-associated lipocalin cAMP cyclic adenosine monophosphate PBMCs peripheral blood mononuclear cells CKD chronic kidney disease pFSGS primary focal segmental glomerulosclerosis CRISPR/Cas9 clustered regularly interspaced short palindromic repeats/CRISPR-associated protein 9 ROS reactive oxygen species ECM extracellular matrix scRNA-seq single-cell RNA sequencing ESC embryonic stem cell TCBs T cell bispecific antibodies ESRD end-stage renal disease TGF-1 transforming growth factor beta 1 EZH2 enhancer of zeste homolog 2 VEGF vascular endothelial growth factor Glossary References 1
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Although direct neo-N-terminal fragments of caspase-1 were not enriched in the TMT-TAILS dataset, potentially due to labeling inefficiencies, we observed significant depletion of peptides spanning the X(PC) cleavage site in the active protease-treated samples, providing strong evidence of on-target cleavage (Extended Data Fig
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