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The resin was washed with 10 resin volumes of the washing buffer (20 mM Hepes pH 7.5, 400 mM NaCl, 20 mM Imidazole) and eluted with the elution buffer (20 mM Hepes pH 7.5, 400 mM NaCl, 400 mM Imidazole)

Under that plan, the primary endpoint was redefined as percent change rather than point change, and the cardiac endpoint became a ranked change

The relationship is that TB-500 is a truncated active fragment of Thymosin Beta-4 and identified through systematic deletion analysis as the minimal sequence retaining the full biological activity of the intact protein in wound healing, cell migration, and angiogenesis assays

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Whey proteins quality is, however, important