The IGF-1R and IR are highly homologous, especially in the TK domain in which they share 84% amino-acid identities
To exclude the possibility that GSH is cleaved by enzymes to release glutamate for Ca signal propagation, we employed GGsTop, a well-known chemical inhibitor that specifically targets gamma-glutamyl transpeptidase (GGT), the enzyme responsible for converting GSH to Glu 26
Swirl the vial gently for 3060 seconds until fully dissolved
- Pike CJ, Burdick D, Walencewicz AJ, Glabe CG, Cotman CW
In cardiac cells, the physical proximity between mitochondria and the sarcoplasmic reticulum (SR, a specific type of ER in heart and skeletal muscle cells) allows the exchange of Ca 2+ in local microdomains, providing rapid Ca 2+ buffering