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However, completely abolishing BACE1 by knocking out the BACE1 gene has adverse effects on recovery after SCI such as delayed myelination and decreased myelin thickness (hypomyelination) due to a dysregulation of neuregulin-1 type III, an EGF-like growth factor (Hu et al., 2006

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The supernatant was collected and filtered through a 0.45-m filter (Millipore SLHAR33SS)