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The reduction of dehydroascorbate may be non-enzymatic or catalysed by proteins with dehydroascorbate reductase activity, such as glutathione S -transferase omega 1 or glutaredoxins
Optimal results require consistent daily dosing combined with regular exercise and adequate protein intake
For standard doses between 2.5 mg and 10 mg, the 1 mL (100 unit) syringe works perfectly
The standard daily dose is 2,000 to 2,500 mcg of total blend, injected subcutaneously once daily
Tissues were counterstained with haematoxylin, mounted with Permount mounting medium (Fisher Scientific, SP15500) and coverslipped for imaging
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