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Endocrinology 150:17121722 Kerr BD, Flatt PR, Gault VA (2010) (D-nn2)Oxm[mPEG-PAL]: a novel chemically modified analogue of oxyntomodulin with antihyperglycaemic, insulinotropic and anorexigenic actions

The fusion protein is captured by immobilized glutathione and impurities are washed away

Flick the syringe gently with your finger to move bubbles to the top (near the needle), then push the plunger slightly to expel them

For treatments, similar to our imaging conditions, cells were treated with 500 M H 2 O 2 for 10 min

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