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Next, all samples were precipitated and washed using a standard Methanol/Chloroform protocol adapted from previous references 80

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Interactions Between GLP-1 Medications and Levothyroxine For people taking levothyroxine for hypothyroidism who start GLP-1 therapy, potential interactions exist that may require monitoring and dose adjustments

Adjustments may include pausing treatment, extending time at the current dose, or switching to an alternative therapy under professional guidance

Addition of MBB (30 l of stock solution (1mM)) started the reaction and was followed as above