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What you control by choosing your diluent volume is the concentration how much peptide sits in each milliliter of finished solution

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2.4 High-performance liquid chromatography-tandem mass spectrometry analysis of floret ergothioneine concentrations EGT was extracted from the florets and analyzed by high-performance liquid chromatography-tandem mass spectrometry (HPLC-MS/MS) as described by v / v ) HPLC-grade methanol, and shaken on a vortex platform for 10 min

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