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The disulfide bond between Pt GSTF8 residue C13 and GSH was reduced on-column with 20 column volumes of rPPS buffer (20 mM potassium phosphate, 200 mM NaCl, 10 mM DTT, pH 7.4), at a flow rate of 5 ml min 1 and the column was then washed with 40 column volumes of PPS buffer at a flow rate of 5 ml min 1 to remove free GSH
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The interaction between lithium orotate and glutathione synthesis introduces another layer of complexity to formulation strategies
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