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Taking 1 mL working solution as an example, add 100 L DMSO stock solution ( 8.3 mg/mL ) to 900 L 20% SBE--CD in Saline, and mix evenly
Allowing both the peptide vial and BAC water to naturally reach room temperature before reconstitution can improve handling and help the lyophilized cake dissolve more evenly
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The multiple-tube method, also called most probable number, splits the sample across several tubes and uses how many show growth to estimate the count
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