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Protein and lipid pellets were discarded, while 10 L injections of supernatants were analyzed via a Vanquish UHPLC system (Thermo Fisher Scientific) coupled to a Q Exactive mass spectrometer (Thermo Fisher Scientific) utilizing an untargeted acquisition as previously reported 18

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Is there evidence supporting maintenance on a lower dose rather than complete discontinuation

Clinical trials document typical outcomes of 15-20% body weight loss over 12-18 months, representing substantial, well-documented metabolic effects